Generator

Part:BBa_K1021029

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-09-13)

PtrpC+nptII+TtrpC

This is the complete generator for constitutive expression of the neomycin phosphotransferase gene in fungi.

Usage and Biology

  • This part was constructed from BBa_K1021007, BBa_K1021001, and BBa_K1021008.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1171
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1004
    Illegal NgoMIV site found at 1669
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1385
    Illegal SapI.rc site found at 853
    Illegal SapI.rc site found at 1063


[edit]
Categories
Parameters
None